d10a8 fluidigm 3147005a  (fluidigm)

Journal: Current protocols in molecular biology

Article Title: Preparing Viable Single Cells from Human Tissue and Tumors for Cytomic Analysis Current Protocols in Molecular Biology UNIT 25C.1

doi: 10.1002/cpmb.37

Figure Lengend Snippet: Tissue-specific antibody panels

Article Snippet: This protocol was adapted from previously established fluorescence flow cytometry protocols ( Krutzik et al., 2005 ; Krutzik et al., 2004 ; Leelatian et al., 2015 ; McLaughlin et al., 2008 ). table ft1 table-wrap mode="anchored" t5 caption a7 Metal Antibody Clone Working conc (μg/mL) Dilution Volume to use in 100 μL stain (μL) Sample type Staining condition Ton Glio Mel Surf Sap MeOH 141Pr HLA-ABC W3-32 Fluidigm 1:200 0.5 ✓ ✓ 142Nd cCasp3 D3E9 Fluidigm 1:200 0.5 ✓ ✓ ✓ ✓ 144Nd CD11b ICRF44 Fluidigm 1:200 0.5 ✓ ✓ 145Nd CD4 RPA-T4 Fluidigm 1:200 0.5 ✓ ✓ CD31 WM59 Fluidigm 1:200 0.5 ✓ ✓ ✓ 146Nd IgD IA6-2 Fluidigm 1:200 0.5 ✓ ✓ CD64 10.1 Fluidigm 1:200 0.5 ✓ ✓ CD8a RPA-T8 Fluidigm 1:200 0.5 ✓ ✓ 147Sm β-catenin D10A8 Fluidigm 1:200 0.5 ✓ ✓ 148Nd CD16 3G8 Fluidigm 1:200 0.5 ✓ ✓ 149Sm CD45RO UCHL1 Fluidigm 1:200 0.5 ✓ ✓ ✓ ✓ 152Sm TUJ1 TUBB3 50 1:100 1 ✓ ✓ 153Eu CD45RA HI100 Fluidigm 1:200 0.5 ✓ ✓ S100B 19-S100B 100 1:100 1 ✓ ✓ ✓ 154Gd CD45 HI30 Fluidigm 1:400 0.25 ✓ ✓ ✓ ✓ 155Gd CD27 L128 Fluidigm 1:100 1 ✓ ✓ 156Dy Vimentin RV202 Fluidigm 1:200 0.5 ✓ ✓ 159Tb CD11c Bu15 Fluidigm 1:200 0.5 ✓ ✓ CD49F GoH3 100 1:100 1 ✓ ✓ 160Gd CD14 M5E2 Fluidigm 1:200 0.5 ✓ ✓ 161Dy CD19 HIB19 100 1:100 1 ✓ ✓ PDGFRα 16A1 200 1:100 1 ✓ ✓ Cytokeratin C-11 Fluidigm 1:200 0.5 ✓ ✓ 162Dy c-MET L6E7 100 1:100 1 ✓ ✓ 163Dy SOX2 O30-678 100 1:100 1 ✓ ✓ ✓ 166Er CD24 ML5 Fluidigm 1:200 0.5 ✓ ✓ 167Er CD38 HIT2 Fluidigm 1:200 0.5 ✓ ✓ 168Er CD8 SK1 Fluidigm 1:200 0.5 ✓ ✓ Nestin 10C2 100 1:100 1 ✓ ✓ 169Tm CD44 BJ18 100 1:100 1 ✓ ✓ ✓ 170Er CD3 SP Fluidigm 1:200 0.5 ✓ ✓ ✓ ✓ 171Yb CD68 Y1/82A Fluidigm 1:200 0.5 ✓ ✓ GFAP 1B4 25 1:100 1 ✓ ✓ 172Yb IgM MHM-88 Fluidigm 1:200 0.5 ✓ ✓ 173Yb αSMA Ab54723 50 1:100 1 ✓ ✓ ✓ 174Yb HLA-DR L243 Fluidigm 1:200 0.5 ✓ ✓ ✓ ✓ 175Lu CD56 HCD56 50 1:100 1 ✓ ✓ ✓ ✓ 176Yb Histone H3 D1H2 Fluidigm 1:200 0.5 ✓ ✓ ✓ ✓ Open in a separate window Conc = concentration; Ton = tonsil; Glio = glioma; Mel = melanoma; Surf = surface; Sap = saponin; MeOH = post-methanol Fluidigm = use antibodies provided by Fluidigm Tissue-specific antibody panels

Techniques: Staining